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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Isolation and culture of oculomotor, trochlear, and spinal motor neurons from prenatal Isl MN :GFP transgenic mice
doi: 10.3791/60440
Figure Lengend Snippet: Table of Materials
Article Snippet: Horse serum, heat inactivated, New Zealand origin Thermo Fisher Scientific 26050-070 IBMX Tocris Cookson 2845 Isobutylmethylxanthine Laminin Thermo Fisher Scientific 23017-015 Leibovitz’s L15 medium Thermo Fisher Scientific 11415064 2-Mercaptoethanol Sigma-Aldrich M6250 Micro Dissecting Scissors Roboz Surgical Instrument RS-5913 Micro Knife 4.75” 1.7 × 27 mm blade Roboz Surgical Instrument RS-6272 Moria Mini Perforated Spoon Fine Science Tools 10370-19 mouse monoclonal antibody to neuronal class III β-tubulin (TUBB3) BioLegend 801202 1:500, TUJ1 Nikon Perfect Focus Eclipse Ti live cell fluorescence microscope and Elements software Nikon - Differential interference contrast images and immunocytochemistry images of the cell cultures were captured with these equipments Nitric Acid 90%, Fuming (Certified ACS) Fisher Scientific A202-212 For rinsing coverslips Olympus 1.7ml Microtubes, Clear
Techniques: Flow Cytometry, Cell Culture, Microscopy, Fluorescence, Software, Immunocytochemistry, Dissection